As a special form of light microscopy, fluorescence spectroscopy is an analytical measuring method for the characterization of emission properties of fluorescent materials. Typical fluorophores can be found in nature, for example in the form of atoms, molecules, ions or semiconductor nanoparticles (quantum dots). The fluorescent object to be investigated is stimulated to glow with a laser and the resulting fluorescence signal is collected via an objective (Zeiss LD Plan Neofluar) with 63x magnification and a numerical aperture of 0.75 and imaged on a CCD camera (Andor Technology, Newton CCD). With the additional possibility of scanning the sample by means of piezo nanopositioning technology, both time- and spatially-resolved spectroscopy can be performed.
- Fluorescence spectroscopy (spatially- and time-resolved)
- Confocal microscopy
- Ultraviolet spectroscopy